Homologous Recombination in Rat Germline Stem Cells1
نویسندگان
چکیده
منابع مشابه
Designing E1 Deleted Adenoviral Vector by Homologous Recombination
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The CRISPR/Cas9 system has been applied in a large number of animal and plant species for genome editing. In chickens, CRISPR has been used to knockout genes in somatic tissues, but no CRISPR-mediated germline modification has yet been reported. Here we use CRISPR to target the chicken immunoglobulin heavy chain locus in primordial germ cells (PGCs) to produce transgenic progeny. Guide RNAs wer...
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ژورنال
عنوان ژورنال: Biology of Reproduction
سال: 2011
ISSN: 0006-3363,1529-7268
DOI: 10.1095/biolreprod.111.090837